implementation of two-component time-dependent density functional theory Search Results


pyroptosis compound library  (Selleck Chemicals)
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Selleck Chemicals pyroptosis compound library
Micheliolide (MCL) inhibits radiation-induced NLRP3 inflammasome-dependent <t>pyroptosis</t> in vitro (A,B) Expression of NLRP3 in control (Ctl) and NLRP3-silenced (si-NLRP3) cells was detected by western blotting and quantitative real-time polymerase chain reaction (C) Cell viability was significantly increased by MCL (10 μM) treatment and NLRP3 knockout manner following treatment with radiation (10 Gy) for 48 h (D) LDH release was significantly decreased by MCL (10 μM) treatment and NLRP3 knockout manner following treatment with radiation (10 Gy) for 48 h (E) Cell culture supernatant IL-1β levels (F) Cell culture supernatant IL-18 levels (G,H) Expression of pro-casp1, casp1 p20, NLRP3, GSDMD, and GSDMD-N in si-NLRP3 cells and si-NC cells treated with MCL (10 μM) following irradiation (10 Gy) (I) Representative propidium iodide staining fluorescence image (J) Representative flow cytometry scatter plots. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-tailed Student’s t-test. IL, interleukin; NLRP3, nucleotide binding domain leucine-rich repeat-containing receptor-pyrin domain containing three; GSDMD, gasdermin D.
Pyroptosis Compound Library, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Micheliolide (MCL) inhibits radiation-induced NLRP3 inflammasome-dependent pyroptosis in vitro (A,B) Expression of NLRP3 in control (Ctl) and NLRP3-silenced (si-NLRP3) cells was detected by western blotting and quantitative real-time polymerase chain reaction (C) Cell viability was significantly increased by MCL (10 μM) treatment and NLRP3 knockout manner following treatment with radiation (10 Gy) for 48 h (D) LDH release was significantly decreased by MCL (10 μM) treatment and NLRP3 knockout manner following treatment with radiation (10 Gy) for 48 h (E) Cell culture supernatant IL-1β levels (F) Cell culture supernatant IL-18 levels (G,H) Expression of pro-casp1, casp1 p20, NLRP3, GSDMD, and GSDMD-N in si-NLRP3 cells and si-NC cells treated with MCL (10 μM) following irradiation (10 Gy) (I) Representative propidium iodide staining fluorescence image (J) Representative flow cytometry scatter plots. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-tailed Student’s t-test. IL, interleukin; NLRP3, nucleotide binding domain leucine-rich repeat-containing receptor-pyrin domain containing three; GSDMD, gasdermin D.

Journal: Frontiers in Pharmacology

Article Title: Autophagy Induced by Micheliolide Alleviates Acute Irradiation-Induced Intestinal Injury via Inhibition of the NLRP3 Inflammasome

doi: 10.3389/fphar.2021.773150

Figure Lengend Snippet: Micheliolide (MCL) inhibits radiation-induced NLRP3 inflammasome-dependent pyroptosis in vitro (A,B) Expression of NLRP3 in control (Ctl) and NLRP3-silenced (si-NLRP3) cells was detected by western blotting and quantitative real-time polymerase chain reaction (C) Cell viability was significantly increased by MCL (10 μM) treatment and NLRP3 knockout manner following treatment with radiation (10 Gy) for 48 h (D) LDH release was significantly decreased by MCL (10 μM) treatment and NLRP3 knockout manner following treatment with radiation (10 Gy) for 48 h (E) Cell culture supernatant IL-1β levels (F) Cell culture supernatant IL-18 levels (G,H) Expression of pro-casp1, casp1 p20, NLRP3, GSDMD, and GSDMD-N in si-NLRP3 cells and si-NC cells treated with MCL (10 μM) following irradiation (10 Gy) (I) Representative propidium iodide staining fluorescence image (J) Representative flow cytometry scatter plots. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-tailed Student’s t-test. IL, interleukin; NLRP3, nucleotide binding domain leucine-rich repeat-containing receptor-pyrin domain containing three; GSDMD, gasdermin D.

Article Snippet: 441 compounds in Pyroptosis Compound Library (Selleck) was used in order to screening radiation protective drugs.

Techniques: In Vitro, Expressing, Control, Western Blot, Real-time Polymerase Chain Reaction, Knock-Out, Cell Culture, Irradiation, Staining, Fluorescence, Flow Cytometry, Two Tailed Test, Binding Assay